Constructing Bacterial Membrane-Mimicking Vesicle Models as a Tool to Monitor Uptake of Molecules

Observing basic biological phenomenon in prokaryotic organisms encounter many complications and challenges. With the advancement of synthetic biology recent years, several cell-mimicking tools have emerged. Hence, need to engineer robust models that could assist bottom up research has become more demanding. Currently Giant Unilamellar Vesicles (GUVs) are becoming most preferred approach. In this work we construct GUV mimic inner membrane Gram-negative Gram-positive bacteria under physiological salt pH conditions. We used an E-coli lipid extract for (GN) model a ternary mixture mimicking Staphylococcus aureus (GP) model. successfully able control size vesicles further micro-compartments with vesicle-in-vesicle configurations. The functionality is tested by monitoring transport two sensitive antibiotics across membrane. Both show dependent difference antibiotic permeability. More importantly, based on composition remarkable permeability observed between models, following which proposed permeation Furthermore, vesicle be as scaffold study antimicrobial peptide activity. uptake small molecules pore embedded reveal vesicles. engineered here not only crucial understanding mechanisms, but also form building block towards construction protocell.